Kuwait University
COLLEGE OF ENGINEERING & PETROLEUM

Principle:
In HPLC, the liquid (mobile phase) is forced through a column of the stationary phase (usually a tube packed with small round particles with a certain surface chemistry) by pumping at high pressure. The sample to be analyzed is introduced in a small volume to the stream of mobile phase and is retarded by specific chemical or physical interactions with the stationary phase as it traverses the length of the column. The amount of retardation depends on the nature of the sample content, stationary phase and mobile phase composition. The time at which a specific compound elutes (comes out of the end of the column) is called the retention time and is considered a reasonably unique identifying characteristic of that given compound.

Applications:

  • Isolation and/or Identification of a compound.
  • Purification of a sample.
  • Determination of a composition of a sample.


Specifications:

  • Mobile phase flow arte =  0.01 to 20.0 mL/min,
  • 4 solvents can be proportioned from 1 to 100% ratio
  • Isocratic or gradient mode.
  • Choice of various sample loop sizes for injection.
  • Detector Refractive Index Linear dynamic range 5x10-3 to 5x10-8
  • Fully PC controlled of flow rate, solvent composition and sample injection.


Manufacturer and Model No.

Manufacturer:Thermo Scientific
Model No.: Finnigan Surveyor

 















Location: Bldg No. 8Kh, 209
Contact: Ext. 3621

High-Performance Liquid Chromatograph